端基官能化聚乙烯吡咯烷酮用于侧流装置测试线中的配体显示《ACS Polymers Au》

端基官能化聚乙烯吡咯烷酮用于侧流装置测试线中的配体显示

摘要

侧流设备是快速（通常成本较低）的护理诊断方式——典型的例子是家庭怀孕测试。测试线（固定相）通常是通过物理吸附抗体来制备的，抗体与分析物/抗原（如病毒、毒素或激素）结合。然而，检测单元没有抗体的内在要求，并且结合其他配体类可能带来新的功能或检测能力。为了使其他（非蛋白）配体能够部署在侧流装置中，它们必须作为共轭物物理吸附到固定相，该共轭物目前将是一种高分子量载体蛋白，需要（具有挑战性的）化学选择性修饰和纯化。在此，我们证明聚乙烯吡咯烷酮（PVP）由于其独特的水溶性平衡（用于印刷）和与硝化纤维素固定相的粘附性，是聚合物、无蛋白测试线的候选产品。通过RAFT聚合制备端基官能化PVP，并将生物素和半乳糖胺的模型捕获配体安装在PVP上，然后固定在硝化纤维素上。该聚合物测试线使用链霉亲和素和大豆凝集素在流通和全侧流两种形式下进行了验证，是第一个证明“全聚合物”方法安装捕获单元的方法。这项工作说明了高分子支架作为小分子捕获剂的锚定剂在下一代强劲的模块化侧流装置中的潜力，并且高分子工程可能提供真正的益处。

关键词：侧流分析, 聚合物, RAFT, 聚糖, 诊断, 生物传感

End-Functionalized Poly(vinylpyrrolidone) for Ligand Display in Lateral Flow Device Test Lines

Alexander N. Baker, Thomas R. Congdon, Sarah-Jane Richards, Panagiotis G. Georgiou, Marc Walker, Simone Dedola, Robert A. Field, and Matthew I. Gibson*

Department of Chemistry, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, U.K.

Warwick Medical School, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, U.K.

ACS Polym. Au 2022, 2, 2, 69–79

Publication Date: November 12, 2021

https://doi.org/10.1021/acspolymersau.1c00032

Abstract

Lateral flow devices are rapid (and often low cost) point-of-care diagnostics─the classic example being the home pregnancy test. A test line (the stationary phase) is typically prepared by the physisorption of an antibody, which binds to analytes/antigens such as viruses, toxins, or hormones. However, there is no intrinsic requirement for the detection unit to be an antibody, and incorporating other ligand classes may bring new functionalities or detection capabilities. To enable other (nonprotein) ligands to be deployed in lateral flow devices, they must be physiosorbed to the stationary phase as a conjugate, which currently would be a high-molecular-weight carrier protein, which requires (challenging) chemoselective modifications and purification. Here, we demonstrate that poly(vinylpyrrolidone), PVP, is a candidate for a polymeric, protein-free test line, owing to its unique balance of water solubility (for printing) and adhesion to the nitrocellulose stationary phase. End-functionalized PVPs were prepared by RAFT polymerization, and the model capture ligands of biotin and galactosamine were installed on PVP and subsequently immobilized on nitrocellulose. This polymeric test line was validated in both flow-through and full lateral flow formats using streptavidin and soybean agglutinin and is the first demonstration of an “all-polymer” approach for installation of capture units. This work illustrates the potential of polymeric scaffolds as anchoring agents for small-molecule capture agents in the next generation of robust and modular lateral flow devices and that macromolecular engineering may provide real benefit

KEYWORDS: lateral flow assay, polymers, RAFT, glycans, diagnostics, biosensing

来源：《ACS Polymers Au》

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