| HS-PEG-COOH (Thiol PEG Carboxylic acid) |
|
| PBL-8073 | HS-PEG-COOH, MW 1k |
| PBL-8074 | HS-PEG-COOH, MW 2k |
| PBL-8075 | HS-PEG-COOH, MW 3.4k |
| PBL-8076 | HS-PEG-COOH, MW 5k |
| PBL-8077 | HS-PEG-COOH, MW 10k |
Description:
HS-PEG-COOH is a linear heterobifunctional PEGylation reagent with a thiol and a carboxylic acid. It is a useful crosslinking or bioconjugation reagent with a PEG spacer. Thiol, or SH, sulfhydryl, or mercapto group selectively reacts with maleimide, OPSS, vinylsulfone and transition metal surface including gold, silver, etc.
References:
1. High-Resolution Single-Molecule Recognition Imaging of the Molecular Details of Ricin−Aptamer Interaction, J. Phys. Chem. B 2012, 5316−5322, Text.
2. Transport of nanoparticles through the blood–brain barrier for imaging and therapeutic applications, Nanoscale, 2014, 6, 2146,Text.
3. New optical method for enhanced detection of colon cancer by capsule endoscopy, Nanoscale, 2013, 5, 9806, Text.
4. Single-Molecule Force Spectroscopy of the Aplysia Cell Adhesion Molecule Reveals Two Homophilic Bonds. Biophysical Journal 2012; 103: 649–657, Text.
5. The Effect of Nanoparticle Size on Cellular Binding Probability, Journal of Atomic, Molecular, and Optical Physics. 2012, 404536, Text.
6. Inducing Stem Cell Myogenesis using NanoScript.ACS Nano, 2015, 9 (7), pp 6909–6917. Text.
7. The effect of nanoparticle size on the ability to cross the blood–brain barrier: an in vivo study, Nanomedicine, 2017, 12(13), 0022. Text.
8. Investigating the Effect of Substrate Materials on Wearable Immunoassay Performance, Langmuir, 2017, 33 (3), 773–782, Text.
Q: What are the differences among your PEG NHS ester & PEG Acid products?
A: We offer a variety of PEG Acid and PEG NHS ester. These NHS esters react with primary and secondary amine to form a stable amide linkage. This reaction may be carried out in an organic solution with an organic base or may be carried out in an aqueous solution at or around neutral pH or slightly acidic or basic. To PEGylate proteins or nanoparticles, it often requires a large excess of PEG NHS esters due to the competitive hydrolysis reaction in an aqueous solution. Stability of NHS esters is compared by their hydrolysis half-life (T1/2). To mitigate the competitive hydrolysis reaction, organic solvents such as amine-free, anhydrous DMF and DMSO may be used in combination with an aqueous buffer solution.
PEG NHS esters | Structural characteristics | Amine reactivity | Stability |
|---|
| Type A: SCM | Methylene (CH2) linkage between PEG and NHS ester | Highly reactive | Hydrolysis half-life: less than five minutes. It often requires the use of a large excess of PEG reagents. |
| Type B: SG | C4 aliphatic ester linkage between PEG and NHS ester | Very reactive | Hydrolysis half-life: around 20 minutes. |
| Type C: SS | C3 aliphatic ester linkage between PEG and NHS ester | Very reactive | Hydrolysis half-life: around 10 minutes. |
| Type D: GAS | C4 aliphatic amide linkage between PEG and NHS ester | Very reactive | Hydrolysis half-life: around 20 minutes. |
| Type E: SAS | C3 aliphatic amide linkage between PEG and NHS ester | Very reactive | Hydrolysis half-life: around 10 minutes. |
SCM: Succinimidyl Carboxyl Methyl ester; SG: Succinimidyl Glutarate ester; SS: Succinimidyl Succinate ester; GAS: GlutarAmide Succinimidyl ester; SAS: SuccinAmide Succinimidyl ester. Note: hydrolysis half-life depends on pH, temperature and other factors of the testing conditions.
| PEG acids | Structural characteristics |
|---|
| Type A: AA | Methylene (CH2) linkage between PEG and acid (COOH) |
| Type B: GA | C4 aliphatic ester linkage between PEG and acid (COOH) |
| Type C: SA | C3 aliphatic ester linkage between PEG and acid (COOH) |
| Type D: GAA | C4 aliphatic amide linkage between PEG and acid (COOH) |
| Type E: SAA | C3 aliphatic amide linkage between PEG and acid (COOH) |
AA: Acetic Acid, also called CM - Carboxyl Methyl; GA: Glutaric Acid; SA: Succinic Acid; GAA: GlutarAmide Acid; SAS: SuccinAmide Acid.
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